› 2c4b

X-ray diffraction
1.3Å resolution

Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A

Released:
DOI: 10.2210/pdb2c4b/pdb
PDB entry 2c4b coloured by chain, front view.
PDB entry 2c4b coloured by chain, side view.
PDB entry 2c4b coloured by chain, top view.
Source organisms:
Primary publication:
Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI.
Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW
J Mol Biol 356 1-8 (2006)
PMID: 16337652

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-523744 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease; Trypsin inhibitor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 143 amino acids
Theoretical weight: 15.73 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P00648 (Residues: 49-157; Coverage: 89%)
  • Canonical: P82409 (Residues: 6-21; Coverage: 47%)
  • Canonical: P12071 (Residues: 16-29; Coverage: 47%)
Sequence domains:
Structure domains: Microbial ribonucleases

Ligands and Environments

7 bound ligands:
Ligand 2PE 4 x 2PE
Ligand GOL 1 x GOL
Ligand EDO 10 x EDO
Ligand FMT 3 x FMT
Ligand SO4 17 x SO4
Ligand UNX 15 x UNX
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: C2221
Unit cell:
a: 74.02Å b: 217.71Å c: 58.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.132 0.164
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Barnase and binase: twins with distinct fates.
Ulyanova et al. (2011)
1 more

1 mention without citation

PredSTP: a highly accurate SVM based model to predict sequential cystine stabilized peptides.
Islam et al. (2015)