› 2c4l

X-ray diffraction
2.15Å resolution

Structure of Neuraminidase Subtype N9 Complexed with 30 MM Sialic Acid (NANA, NEU5AC), Crystal Soaked for 24 Hours at 291 K and Finally Backsoaked for 30 Min in a Cryoprotectant Solution which did not contain NEU5AC

Released:
DOI: 10.2210/pdb2c4l/pdb
PDB entry 2c4l coloured by chain, front view.
PDB entry 2c4l coloured by chain, side view.
PDB entry 2c4l coloured by chain, top view.
Source organism: Influenza A virus
Entry authors: Rudino-Pinera E, Tunnah P, Crennell SJ, Webster RG, Laver WG, Garman EF

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137058 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neuraminidase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.72 KDa
Source organism: Influenza A virus
Expression system: Gallus gallus
UniProt:
  • Canonical: P03472 (Residues: 83-470; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
4 bound ligands:
Ligand SIA 2 x SIA
Ligand NAG 5 x NAG
Ligand BMA 5 x BMA
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200H
Spacegroup: I432
Unit cell:
a: 181.178Å b: 181.178Å c: 181.178Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.155 0.196
Expression system: Gallus gallus

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