› 2c76

X-ray diffraction
1.7Å resolution

Functional Role of the Aromatic Cage in Human Monoamine Oxidase B: Structures and Catalytic Properties of Tyr435 Mutant Proteins

Released:
DOI: 10.2210/pdb2c76/pdb
PDB entry 2c76 coloured by chain, front view.
PDB entry 2c76 coloured by chain, side view.
PDB entry 2c76 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Functional role of the "aromatic cage" in human monoamine oxidase B: structures and catalytic properties of Tyr435 mutant proteins.
Li M, Binda C, Mattevi A, Edmondson DE
Biochemistry 45 4775-84 (2006)
PMID: 16605246

Function and Biology Details

Reactions catalysed: 
RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)
RCH(2)NHR' + H(2)O + O(2) = RCHO + R'NH(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150973 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amine oxidase [flavin-containing] B Chains: A, B
Molecule details ›
Chains: A, B
Length: 520 amino acids
Theoretical weight: 58.86 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P27338 (Residues: 1-520; Coverage: 100%)
Gene name: MAOB
Sequence domains: Flavin containing amine oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
Ligand RSA 2 x RSA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C222
Unit cell:
a: 131.242Å b: 223.063Å c: 86.361Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.213
Expression system: Komagataella pastoris

Quick links

Citations

10 review citations

Molecular and mechanistic properties of the membrane-bound mitochondrial monoamine oxidases.
Edmondson et al. (2009)
9 more

1 mention without citation

Survey of Drug Oxidation Activities in Hepatic and Intestinal Microsomes of Individual Common Marmosets, a New Nonhuman Primate Animal Model.
Uehara et al. (2019)