› 2ca9

X-ray diffraction
2.05Å resolution

apo-NIKR from helicobacter pylori in closed trans-conformation

Released:
DOI: 10.2210/pdb2ca9/pdb
PDB entry 2ca9 coloured by chain, front view.
PDB entry 2ca9 coloured by chain, side view.
PDB entry 2ca9 coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Structural basis of the nickel response in Helicobacter pylori: crystal structures of HpNikR in Apo and nickel-bound states.
Dian C, Schauer K, Kapp U, McSweeney SM, Labigne A, Terradot L
J Mol Biol 361 715-30 (2006)
PMID: 16872629

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative nickel-responsive regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 148 amino acids
Theoretical weight: 17.17 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O25896 (Residues: 1-148; Coverage: 100%)
Gene name: HP_1338
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FMT 7 x FMT
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6122
Unit cell:
a: 71.055Å b: 71.055Å c: 229.023Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.199 0.25
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
12 more

5 mentions without citation

Allosteric control of metal-responsive transcriptional regulators in bacteria.
Baksh et al. (2020)
4 more