› 2clv

X-ray diffraction
1.9Å resolution

MHC Class I Natural Mutant H-2Kbm8 Heavy Chain Complexed With beta-2 Microglobulin and pBM8 peptide

Released:
DOI: 10.2210/pdb2clv/pdb
PDB entry 2clv coloured by chain, front view.
PDB entry 2clv coloured by chain, side view.
PDB entry 2clv coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Distinct orientation of the alloreactive monoclonal CD8 T cell activation program by three different peptide/MHC complexes.
Auphan-Anezin N, Mazza C, Guimezanes A, Barrett-Wilt GA, Montero-Julian F, Roussel A, Hunt DF, Malissen B, Schmitt-Verhulst AM
Eur J Immunol 36 1856-66 (2006)
PMID: 16761314

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135063 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 279 amino acids
Theoretical weight: 32.08 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01901 (Residues: 22-300; Coverage: 80%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, P
Molecule details ›
Chains: B, P
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
RNA-binding protein 5 Chains: C, M
Molecule details ›
Chains: C, M
Length: 8 amino acids
Theoretical weight: 1.04 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q91YE7 (Residues: 484-491; Coverage: 1%)
Gene names: Luca15, Rbm5

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 67.146Å b: 90.461Å c: 89.032Å
α: 90° β: 111.02° γ: 90°
R-values:
R R work R free
0.212 0.21 0.251
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

7 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
6 more

1 mention without citation

MHC I stabilizing potential of computer-designed octapeptides.
Wisniewska et al. (2010)