› 2cvq

X-ray diffraction
2.08Å resolution

Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH

Released:
DOI: 10.2210/pdb2cvq/pdb
PDB entry 2cvq coloured by chain, front view.
PDB entry 2cvq coloured by chain, side view.
PDB entry 2cvq coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structure of NAD-dependent malate dehydrogenase complexed with NADP(H).
Tomita T, Fushinobu S, Kuzuyama T, Nishiyama M
Biochem Biophys Res Commun 334 613-8 (2005)
PMID: 16009341

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 35.47 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10584 (Residues: 1-327; Coverage: 100%)
Gene name: mdh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
1 bound ligand:
Ligand TRS 4 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 70.29Å b: 85.16Å c: 118.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.247
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

An NADH-tetrazolium-coupled sensitive assay for malate dehydrogenase in mitochondria and crude tissue homogenates.
Luo et al. (2006)
5 more