› 2cwr

X-ray diffraction
1.7Å resolution

Crystal structure of chitin biding domain of chitinase from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb2cwr/pdb
Model geometry
Fit model/data
PDB entry 2cwr coloured by chain, front view.
PDB entry 2cwr coloured by chain, side view.
PDB entry 2cwr coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Tertiary structure and carbohydrate recognition by the chitin-binding domain of a hyperthermophilic chitinase from Pyrococcus furiosus.
Nakamura T, Mine S, Hagihara Y, Ishikawa K, Ikegami T, Uegaki K
J Mol Biol 381 670-80 (2008)
PMID: 18582475

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitinase Chain: A
Molecule details ›
Chain: A
Length: 103 amino acids
Theoretical weight: 10.96 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U1H5 (Residues: 258-358; Coverage: 14%)
Gene name: PF1233
Sequence domains: Cellulose binding domain
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P43212
Unit cell:
a: 48.827Å b: 48.827Å c: 84.993Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.226
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacterial chitinases and chitin-binding proteins as virulence factors.
Frederiksen et al. (2013)
3 more

7 mentions without citation

Systematic comparison of crystal and NMR protein structures deposited in the protein data bank.
Sikic et al. (2010)
6 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO