› 2d05

X-ray diffraction
2Å resolution

Chitosanase From Bacillus circulans mutant K218P

Released:
DOI: 10.2210/pdb2d05/pdb
PDB entry 2d05 coloured by chain, front view.
PDB entry 2d05 coloured by chain, side view.
PDB entry 2d05 coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
Bacillus circulans MH-K1 chitosanase: amino acid residues responsible for substrate binding.
Fukamizo T, Amano S, Yamaguchi K, Yoshikawa T, Katsumi T, Saito J, Suzuki M, Miki K, Nagata Y, Ando A
J Biochem 138 563-9 (2005)
PMID: 16272568

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitosanase Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 28.96 KDa
Source organism: Niallia circulans
Expression system: Brevibacillus choshinensis
UniProt:
  • Canonical: P33673 (Residues: 43-301; Coverage: 100%)
Gene name: csn
Sequence domains: Glycosyl hydrolase family 46
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 43.3Å b: 128.1Å c: 57.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.213
Expression system: Brevibacillus choshinensis

Quick links

Citations

3 review citations

Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes.
Viens et al. (2015)
2 more

3 mentions without citation

K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions.
Ostrem et al. (2013)
2 more