› 2dff

X-ray diffraction
2.7Å resolution

Crystal structure of Tk-RNase HII(1-204)-C

Released:
DOI: 10.2210/pdb2dff/pdb
PDB entry 2dff coloured by chain, front view.
PDB entry 2dff coloured by chain, side view.
PDB entry 2dff coloured by chain, top view.
Source organism: Thermococcus kodakarensis KOD1
Primary publication:
Conformational contagion in a protein: structural properties of a chameleon sequence.
Takano K, Katagiri Y, Mukaiyama A, Chon H, Matsumura H, Koga Y, Kanaya S
Proteins 68 617-25 (2007)
PMID: 17510955

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130755 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HII Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 23.96 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: O74035 (Residues: 1-206; Coverage: 90%)
Gene names: TK0805, rnhB
Sequence domains: Ribonuclease HII
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 58.525Å b: 67.109Å c: 52.209Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.248 0.299
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Slow unfolding of monomeric proteins from hyperthermophiles with reversible unfolding.
Mukaiyama et al. (2009)
1 more