› 2dfy

X-ray diffraction
1.65Å resolution

Crystal structure of a cyclized protein fusion of LMO4 LIM domains 1 and 2 with the LIM interacting domain of LDB1

Released:
DOI: 10.2210/pdb2dfy/pdb
PDB entry 2dfy coloured by chain, front view.
PDB entry 2dfy coloured by chain, side view.
PDB entry 2dfy coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Stabilization of a binary protein complex by intein-mediated cyclization.
Jeffries CM, Graham SC, Stokes PH, Collyer CA, Guss JM, Matthews JM
Protein Sci 15 2612-8 (2006)
PMID: 17001033

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LIM domain transcription factor LMO4 Chains: C, X
Molecule details ›
Chains: C, X
Length: 195 amino acids
Theoretical weight: 21.06 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61969 (Residues: 18-152; Coverage: 82%)
Gene name: Lmo4
Sequence domains: LIM domain
Structure domains: Cysteine Rich Protein

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3
Unit cell:
a: 61.333Å b: 61.333Å c: 93.019Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.167 0.194
Expression system: Escherichia coli

Quick links

Citations

4 review citations

LIM-domain-only proteins in cancer.
Matthews et al. (2013)
3 more

7 mentions without citation

Linkers in the structural biology of protein-protein interactions.
Reddy Chichili et al. (2013)
6 more