2e0p

X-ray diffraction
1.6Å resolution

The crystal structure of Cel44A

Released:

Function and Biology Details

Reactions catalysed:
Xyloglucan + H(2)O = xyloglucan oligosaccharides
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159782 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoglucanase J Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 57.69 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli
UniProt:
  • Canonical: P71140 (Residues: 773-1287; Coverage: 33%)
Gene name: celJ
Sequence domains: Glycoside hydrolase family 44
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 47.898Å b: 59.119Å c: 170.539Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.17 0.186
Expression system: Escherichia coli