2e21

X-ray diffraction
2.7Å resolution

Crystal structure of TilS in a complex with AMPPNP from Aquifex aeolicus.

Released:
Source organism: Aquifex aeolicus
Entry authors: Kuratani M, Yoshikawa Y, Sekine S, Ishii T, Shibata R, Bessho Y, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed:
(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H(2)O
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-130535 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA(Ile)-lysidine synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 317 amino acids
Theoretical weight: 37.39 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67728 (Residues: 1-317; Coverage: 100%)
Gene names: aq_1887, tilS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 94.456Å b: 82.231Å c: 109.431Å
α: 90° β: 105.85° γ: 90°
R-values:
R R work R free
0.226 0.222 0.294
Expression system: Escherichia coli BL21(DE3)