› 2e7d

X-ray diffraction
2.2Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dodecamer
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188002 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-regulated surface determinant protein H Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 14.85 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q931P4 (Residues: 539-664; Coverage: 15%)
Gene names: SAV1731, harA, isdH, sasI
Sequence domains: Iron Transport-associated domain
Structure domains: Immunoglobulin-like

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand ACT 1 x ACT
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A, PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: R32
Unit cell:
a: 124.247Å b: 124.247Å c: 119.655Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.24 0.265
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Recent developments in understanding the iron acquisition strategies of gram positive pathogens.
Sheldon et al. (2015)
6 more

6 mentions without citation

Structural basis for multimeric heme complexation through a specific protein-heme interaction: the case of the third neat domain of IsdH from Staphylococcus aureus.
Watanabe et al. (2008)
5 more