› 2ea1

X-ray diffraction
1.8Å resolution

Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE

Released:
DOI: 10.2210/pdb2ea1/pdb
PDB entry 2ea1 coloured by chain, front view.
PDB entry 2ea1 coloured by chain, side view.
PDB entry 2ea1 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Zhou K, Pan J, Padmanabhan S, Lim RW, Lim LW

Function and Biology Details

Reaction catalysed: 
(1a) RNA containing adenosine-cytidine = an (RNA fragment)-3'-cytidine-2',3'-cyclophosphate + a 5'-a hydroxy-adenosine -3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease I Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 27.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P21338 (Residues: 24-268; Coverage: 100%)
Gene names: JW0603, b0611, rna, rnsA
Sequence domains: Ribonuclease T2 family
Structure domains: Ribonuclease T2-like

Ligands and Environments

1 bound ligand:
Ligand GPG 1 x GPG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 43.2Å b: 49.87Å c: 54.52Å
α: 90° β: 96.37° γ: 90°
R-values:
R R work R free
0.231 0.218 0.257
Expression system: Escherichia coli

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