› 2ec9

X-ray diffraction
2Å resolution

Crystal structure analysis of human Factor VIIa , Souluble tissue factor complexed with BCX-3607

Released:
DOI: 10.2210/pdb2ec9/pdb
PDB entry 2ec9 coloured by chain, front view.
PDB entry 2ec9 coloured by chain, side view.
PDB entry 2ec9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Probing the S2 site of factor VIIa to generate potent and selective inhibitors: the structure of BCX-3607 in complex with tissue factor-factor VIIa.
Krishnan R, Kotian PL, Chand P, Bantia S, Rowland S, Babu YS
Acta Crystallogr D Biol Crystallogr 63 689-97 (2007)
PMID: 17505107

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140353 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Factor VII light chain Chain: L
Molecule details ›
Chain: L
Length: 142 amino acids
Theoretical weight: 16.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08709 (Residues: 61-202; Coverage: 32%)
Gene name: F7
Sequence domains:
Factor VII heavy chain Chain: H
Molecule details ›
Chain: H
Length: 254 amino acids
Theoretical weight: 28.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08709 (Residues: 213-466; Coverage: 57%)
Gene name: F7
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Tissue factor Chain: T
Molecule details ›
Chain: T
Length: 75 amino acids
Theoretical weight: 8.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13726 (Residues: 38-112; Coverage: 29%)
Gene name: F3
Sequence domains: Tissue factor
Structure domains: Immunoglobulins
Tissue factor Chain: U
Molecule details ›
Chain: U
Length: 120 amino acids
Theoretical weight: 13.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13726 (Residues: 123-242; Coverage: 46%)
Gene name: F3
Sequence domains: Interferon-alpha/beta receptor, fibronectin type III
Structure domains: Immunoglobulins

Ligands and Environments

4 bound ligands:
Ligand ASO 1 x ASO
Ligand FUC 1 x FUC
Ligand CA 9 x CA
Ligand 24X 1 x 24X
1 modified residue:
Ligand CGU 10 x CGU

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 69.78Å b: 81.41Å c: 125.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.242 0.282
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Epigallocatechin-3-gallate inhibits proliferation and migration of human colon cancer SW620 cells in vitro.
Zhou et al. (2012)
1 more