› 2eir

X-ray diffraction
2.5Å resolution

Design of Disulfide-linked Thioredoxin Dimers and Multimers Through Analysis of Crystal Contacts

Released:
DOI: 10.2210/pdb2eir/pdb
PDB entry 2eir coloured by chain, front view.
PDB entry 2eir coloured by chain, side view.
PDB entry 2eir coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Design of disulfide-linked thioredoxin dimers and multimers through analysis of crystal contacts.
Das M, Kobayashi M, Yamada Y, Sreeramulu S, Ramakrishnan C, Wakatsuki S, Kato R, Varadarajan R
J Mol Biol 372 1278-92 (2007)
PMID: 17727880

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141959 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 108 amino acids
Theoretical weight: 11.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA25 (Residues: 2-109; Coverage: 99%)
Gene names: JW5856, b3781, fipA, trxA, tsnC
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand CU 4 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ DW
Spacegroup: P21
Unit cell:
a: 50.047Å b: 51.042Å c: 88.973Å
α: 90° β: 90.43° γ: 90°
R-values:
R R work R free
0.236 0.232 0.311
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The road to the first, fully active and more stable human insulin variant with an additional disulfide bond.
Vinther et al. (2015)
12 other articles

1 mention without citation

Structural variability of E. coli thioredoxin captured in the crystal structures of single-point mutants.
Noguera et al. (2017)