› 2evw

X-ray diffraction
1.05Å resolution

Crystal structure analysis of a fluorescent form of H-Ras p21 in complex with R-caged GTP

Released:
DOI: 10.2210/pdb2evw/pdb
PDB entry 2evw coloured by chain, ensemble of 2 models, front view.
PDB entry 2evw coloured by chain, ensemble of 2 models, side view.
PDB entry 2evw coloured by chain, ensemble of 2 models, top view.
Source organism: Homo sapiens
Primary publication:
A newly designed microspectrofluorometer for kinetic studies on protein crystals in combination with x-ray diffraction.
Klink BU, Goody RS, Scheidig AJ
Biophys J 91 981-92 (2006)
PMID: 16698776

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: X
Molecule details ›
Chain: X
Length: 166 amino acids
Theoretical weight: 18.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand CAG 1 x CAG
Ligand XY2 1 x XY2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41
Unit cell:
a: 69.3Å b: 69.3Å c: 35.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.156 0.181
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metal Fluorides as Analogues for Studies on Phosphoryl Transfer Enzymes.
Jin et al. (2017)
1 more

6 mentions without citation

phenix.model_vs_data: a high-level tool for the calculation of crystallographic model and data statistics.
Afonine et al. (2010)
5 more