› 2f7c

X-ray diffraction
2.16Å resolution

CatM effector binding domain with its effector cis,cis-muconate

Released:
DOI: 10.2210/pdb2f7c/pdb
PDB entry 2f7c coloured by chain, front view.
PDB entry 2f7c coloured by chain, side view.
PDB entry 2f7c coloured by chain, top view.
Source organism: Acinetobacter baylyi
Primary publication:
Distinct effector-binding sites enable synergistic transcriptional activation by BenM, a LysR-type regulator.
Ezezika OC, Haddad S, Clark TJ, Neidle EL, Momany C
J Mol Biol 367 616-29 (2007)
PMID: 17291527

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139756 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator CatM Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 26.21 KDa
Source organism: Acinetobacter baylyi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07774 (Residues: 81-303; Coverage: 74%)
Gene names: ACIAD1445, catM, catR
Sequence domains: LysR substrate binding domain
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand CCU 1 x CCU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I222
Unit cell:
a: 65.568Å b: 73.108Å c: 102.627Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins.
Maddocks et al. (2008)
3 more

4 mentions without citation

Cell-Wall Recycling of the Gram-Negative Bacteria and the Nexus to Antibiotic Resistance.
Dik et al. (2018)
3 more