› 2f8y

X-ray diffraction
1.55Å resolution

Crystal structure of human Notch1 ankyrin repeats to 1.55A resolution.

Released:
DOI: 10.2210/pdb2f8y/pdb
PDB entry 2f8y coloured by chain, front view.
PDB entry 2f8y coloured by chain, side view.
PDB entry 2f8y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes.
Nam Y, Sliz P, Song L, Aster JC, Blacklow SC
Cell 124 973-83 (2006)
PMID: 16530044

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155479 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Notch 1 intracellular domain Chains: A, B
Molecule details ›
Chains: A, B
Length: 223 amino acids
Theoretical weight: 24.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46531 (Residues: 1905-2126; Coverage: 9%)
Gene names: NOTCH1, TAN1
Sequence domains:
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P65
Unit cell:
a: 97.933Å b: 97.933Å c: 109.068Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.152 0.15 0.187
Expression system: Escherichia coli

Quick links

Citations

80 review citations

The canonical Notch signaling pathway: unfolding the activation mechanism.
Kopan et al. (2009)
79 more

11 mentions without citation

The molecular logic of Notch signaling--a structural and biochemical perspective.
Gordon et al. (2008)
10 more