› 2fe4

X-ray diffraction
2.3Å resolution

The crystal structure of human neuronal Rab6B in its inactive GDP-bound form

Released:
DOI: 10.2210/pdb2fe4/pdb
PDB entry 2fe4 coloured by chain, front view.
PDB entry 2fe4 coloured by chain, side view.
PDB entry 2fe4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of human neuronal Rab6B in the active and inactive form.
Garcia-Saez I, Tcherniuk S, Kozielski F
Acta Crystallogr D Biol Crystallogr 62 725-33 (2006)
PMID: 16790928

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192570 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-6B Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 19.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NRW1 (Residues: 13-174; Coverage: 78%)
Gene name: RAB6B
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand NO3 1 x NO3
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 36.036Å b: 61.125Å c: 66.634Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.222 0.257
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

A survey on prediction of specificity-determining sites in proteins.
Chakraborty et al. (2015)
2 more

1 mention without citation

Using kernelized partial canonical correlation analysis to study directly coupled side chains and allostery in small G proteins.
Soltan Ghoraie et al. (2015)