› 2fhx

X-ray diffraction
1.9Å resolution

Pseudomonas aeruginosa SPM-1 metallo-beta-lactamase

Released:
DOI: 10.2210/pdb2fhx/pdb
PDB entry 2fhx coloured by chain, front view.
PDB entry 2fhx coloured by chain, side view.
PDB entry 2fhx coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure of Pseudomonas aeruginosa SPM-1 provides insights into variable zinc affinity of metallo-beta-lactamases.
Murphy TA, Catto LE, Halford SE, Hadfield AT, Minor W, Walsh TR, Spencer J
J Mol Biol 357 890-903 (2006)
PMID: 16460758

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184473 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Metallo-beta-lactamase type 2 Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.59 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8G9Q0 (Residues: 31-276; Coverage: 99%)
Gene names: CCBH4851_00081, ICEPaeSP_00028, bla SPM-1, blaSPM-1, spm-1
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like
Metallo-beta-lactamase type 2 Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 27.57 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8G9Q0 (Residues: 31-276; Coverage: 99%)
Gene names: CCBH4851_00081, ICEPaeSP_00028, bla SPM-1, blaSPM-1, spm-1
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand AZI 3 x AZI
Ligand EDO 2 x EDO
Ligand CL 1 x CL
2 modified residues:
Ligand CSD 1 x CSD
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM, APS BEAMLINE 19-ID
Spacegroup: R3
Unit cell:
a: 126.936Å b: 126.936Å c: 90.461Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.247
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Carbapenemases: the versatile beta-lactamases.
Queenan et al. (2007)
8 more

10 mentions without citation

Metallo-β-lactamase structure and function.
Palzkill T. (2013)
9 more