› 2h99

X-ray diffraction
1.85Å resolution

Crystal structure of the effector binding domain of a BenM variant (R156H,T157S)

Released:
DOI: 10.2210/pdb2h99/pdb
PDB entry 2h99 coloured by chain, front view.
PDB entry 2h99 coloured by chain, side view.
PDB entry 2h99 coloured by chain, top view.
Source organism: Acinetobacter baylyi ADP1
Primary publication:
Inducer responses of BenM, a LysR-type transcriptional regulator from Acinetobacter baylyi ADP1.
Craven SH, Ezezika OC, Haddad S, Hall RA, Momany C, Neidle EL
Mol Microbiol 72 881-94 (2009)
PMID: 19400783

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator BenM Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 35.61 KDa
Source organism: Acinetobacter baylyi ADP1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O68014 (Residues: 1-304; Coverage: 100%)
Gene names: ACIAD1435, benM, benR
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

4 bound ligands:
Ligand SO4 5 x SO4
Ligand ACT 5 x ACT
Ligand CL 1 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 64.937Å b: 66.506Å c: 117.495Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Peroxide-sensing transcriptional regulators in bacteria.
Dubbs et al. (2012)
3 more

2 mentions without citation

Lessons from high-throughput protein crystallization screening: 10 years of practical experience.
Luft et al. (2011)
1 more