› 2hap

X-ray diffraction
2.5Å resolution

STRUCTURE OF A HAP1-18/DNA COMPLEX REVEALS THAT PROTEIN/DNA INTERACTIONS CAN HAVE DIRECT ALLOSTERIC EFFECTS ON TRANSCRIPTIONAL ACTIVATION

Released:
DOI: 10.2210/pdb2hap/pdb
PDB entry 2hap coloured by chain, front view.
PDB entry 2hap coloured by chain, side view.
PDB entry 2hap coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of HAP1-18-DNA implicates direct allosteric effect of protein-DNA interactions on transcriptional activation.
King DA, Zhang L, Guarente L, Marmorstein R
Nat Struct Biol 6 22-7 (1999)
PMID: 9886287

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-506928 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Heme-responsive zinc finger transcription factor HAP1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 81 amino acids
Theoretical weight: 9.7 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CS82 (Residues: 55-135; Coverage: 6%)
Gene names: CYP1, HAP1
Sequence domains: Fungal Zn(2)-Cys(6) binuclear cluster domain
Structure domains:
DNA (5'-D(*AP*CP*GP*CP*TP*AP*TP*TP*AP*TP*CP*GP*CP*TP*AP*TP*TP*AP*GP*T)-3') Chain: A
Molecule details ›
Chain: A
Length: 20 nucleotides
Theoretical weight: 6.1 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
DNA (5'-D(*AP*CP*TP*AP*AP*TP*AP*GP*CP*GP*AP*TP*AP*AP*TP*AP*GP*CP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.17 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 92.5Å b: 92.5Å c: 86.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.248 0.302
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Nuclear-receptor interactions on DNA-response elements.
Khorasanizadeh et al. (2001)
16 other articles

7 mentions without citation

DNA conformations and their sequence preferences.
Svozil et al. (2008)
6 more