› 2hb0

X-ray diffraction
2.3Å resolution

Crystal Structure of CfaE, the Adhesive Subunit of CFA/I Fimbria of Enterotoxigenic Escherichia coli

Released:
DOI: 10.2210/pdb2hb0/pdb
PDB entry 2hb0 coloured by chain, front view.
PDB entry 2hb0 coloured by chain, side view.
PDB entry 2hb0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A receptor-binding site as revealed by the crystal structure of CfaE, the colonization factor antigen I fimbrial adhesin of enterotoxigenic Escherichia coli.
Li YF, Poole S, Rasulova F, McVeigh AL, Savarino SJ, Xia D
J Biol Chem 282 23970-80 (2007)
PMID: 17569668

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CFA/I fimbrial subunit E Chains: A, B
Molecule details ›
Chains: A, B
Length: 369 amino acids
Theoretical weight: 40.99 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P25734 (Residues: 23-360; Coverage: 94%)
Gene name: cfaE
Sequence domains: CblD like pilus biogenesis initiator
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MLI 3 x MLI
Ligand PEG 4 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6222
Unit cell:
a: 143.346Å b: 143.346Å c: 231.434Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.178 0.201
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Catch-bond mechanism of force-enhanced adhesion: counterintuitive, elusive, but ... widespread?
Sokurenko et al. (2008)
7 more

8 mentions without citation

Structure of CFA/I fimbriae from enterotoxigenic Escherichia coli.
Li et al. (2009)
7 more