› 2hbx

X-ray diffraction
2.5Å resolution

Crystal Structure of alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde-Decarboxylase (ACMSD)

Released:
DOI: 10.2210/pdb2hbx/pdb
PDB entry 2hbx coloured by chain, front view.
PDB entry 2hbx coloured by chain, side view.
PDB entry 2hbx coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Crystal structure of alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase: insight into the active site and catalytic mechanism of a novel decarboxylation reaction.
Martynowski D, Eyobo Y, Li T, Yang K, Liu A, Zhang H
Biochemistry 45 10412-21 (2006)
PMID: 16939194

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182963 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 37.18 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83V25 (Residues: 1-334; Coverage: 100%)
Gene name: nbaD
Sequence domains: Amidohydrolase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

1 bound ligand:
Ligand CO 2 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P42212
Unit cell:
a: 91.569Å b: 91.569Å c: 167.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Asymmetric catalysis with chiral primary amine-based organocatalysts.
Xu et al. (2009)
3 more

2 mentions without citation

Evidence for a dual role of an active site histidine in α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase.
Huo et al. (2012)
1 more