› 2hez

X-ray diffraction
2.5Å resolution

Bifidobacterium longum bile salt hydrolase

Released:
DOI: 10.2210/pdb2hez/pdb
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PDB entry 2hez coloured by chain, front view.
PDB entry 2hez coloured by chain, side view.
PDB entry 2hez coloured by chain, top view.
Source organism: Bifidobacterium longum
Primary publication:
Structural and functional analysis of a conjugated bile salt hydrolase from Bifidobacterium longum reveals an evolutionary relationship with penicillin V acylase.
Kumar RS, Brannigan JA, Prabhune AA, Pundle AV, Dodson GG, Dodson EJ, Suresh CG
J Biol Chem 281 32516-25 (2006)
PMID: 16905539

Function and Biology Details

Reactions catalysed: 
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholan-24-oylglycine + H(2)O = 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + glycine
Chenodeoxycholoyltaurine + H(2)O = chenodeoxycholate + taurine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191912 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bile salt hydrolase/transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 316 amino acids
Theoretical weight: 35.1 KDa
Source organism: Bifidobacterium longum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KK62 (Residues: 2-317; Coverage: 100%)
Gene name: bsh
Sequence domains: Linear amide C-N hydrolases, choloylglycine hydrolase family
Structure domains: Penicillin V Acylase; Chain A

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand OCS 2 x OCS

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU
Spacegroup: P3221
Unit cell:
a: 125.243Å b: 125.243Å c: 117.028Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.174 0.216
Expression system: Escherichia coli BL21(DE3)

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Citations

17 review citations

Consequences of bile salt biotransformations by intestinal bacteria.
Ridlon et al. (2016)
16 more

6 mentions without citation

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
5 more

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