› 2hk8

X-ray diffraction
2.35Å resolution

Crystal structure of shikimate dehydrogenase from aquifex aeolicus at 2.35 angstrom resolution

Released:
DOI: 10.2210/pdb2hk8/pdb
PDB entry 2hk8 coloured by chain, front view.
PDB entry 2hk8 coloured by chain, side view.
PDB entry 2hk8 coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Structural and biochemical analyses of shikimate dehydrogenase AroE from Aquifex aeolicus: implications for the catalytic mechanism.
Gan J, Wu Y, Prabakaran P, Gu Y, Li Y, Andrykovitch M, Liu H, Gong Y, Yan H, Ji X
Biochemistry 46 9513-22 (2007)
PMID: 17649975

Function and Biology Details

Reaction catalysed: 
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130418 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shikimate dehydrogenase (NADP(+)) Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 269 amino acids
Theoretical weight: 30.11 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67049 (Residues: 1-269; Coverage: 100%)
Gene names: aq_901, aroE
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P1
Unit cell:
a: 37.361Å b: 73.96Å c: 192.107Å
α: 94.64° β: 94.16° γ: 91.81°
R-values:
R R work R free
0.214 0.214 0.265
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Mycobacterium tuberculosis Shikimate Pathway Enzymes as Targets for the Rational Design of Anti-Tuberculosis Drugs.
Nunes et al. (2020)
12 other articles

2 mentions without citation

The molecular basis for the broad substrate specificity of human sulfotransferase 1A1.
Berger et al. (2011)
1 more