› 2hsm

X-ray diffraction
3Å resolution

Structural basis of yeast aminoacyl-tRNA synthetase complex formation revealed by crystal structures of two binary sub-complexes

Released:
DOI: 10.2210/pdb2hsm/pdb
PDB entry 2hsm coloured by chain, front view.
PDB entry 2hsm coloured by chain, side view.
PDB entry 2hsm coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis of yeast aminoacyl-tRNA synthetase complex formation revealed by crystal structures of two binary sub-complexes.
Simader H, Hothorn M, Köhler C, Basquin J, Simos G, Suck D
OpenAccess logo Nucleic Acids Res 34 3968-79 (2006)
PMID: 16914447

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155493 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glutamate--tRNA ligase, cytoplasmic Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 22.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P46655 (Residues: 1-191; Coverage: 27%)
Gene names: G0583, GUS1, HRB724, YGL245W
Structure domains:
tRNA-aminoacylation cofactor ARC1 Chain: B
Molecule details ›
Chain: B
Length: 122 amino acids
Theoretical weight: 14 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P46672 (Residues: 1-122; Coverage: 32%)
Gene names: ARC1, G3085, G4P1, YGL105W
Sequence domains: Nuclear-export cofactor Arc1p
Structure domains: Glutathione S-transferase Yfyf (Class Pi); Chain A, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P21
Unit cell:
a: 41.38Å b: 87.46Å c: 52.8Å
α: 90° β: 92.59° γ: 90°
R-values:
R R work R free
0.198 0.195 0.251
Expression system: Escherichia coli

Quick links

Citations

8 review citations

tRNA synthetase: tRNA aminoacylation and beyond.
Pang et al. (2014)
7 more

2 mentions without citation

Personalized Medicine in Mitochondrial Health and Disease: Molecular Basis of Therapeutic Approaches Based on Nutritional Supplements and Their Analogs.
Tragni et al. (2022)
1 more