› 2hw8

X-ray diffraction
2.1Å resolution

Structure of ribosomal protein L1-mRNA complex at 2.1 resolution.

Released:
DOI: 10.2210/pdb2hw8/pdb
PDB entry 2hw8 coloured by chain, front view.
PDB entry 2hw8 coloured by chain, side view.
PDB entry 2hw8 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Structure of the ribosomal protein L1-mRNA complex at 2.1 A resolution: common features of crystal packing of L1-RNA complexes.
Tishchenko S, Nikonova E, Nikulin A, Nevskaya N, Volchkov S, Piendl W, Garber M, Nikonov S
Acta Crystallogr D Biol Crystallogr 62 1545-54 (2006)
PMID: 17139090

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150940 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Large ribosomal subunit protein uL1 Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 24.9 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27150 (Residues: 2-229; Coverage: 100%)
Gene names: rpl1, rplA
Sequence domains: Ribosomal protein L1p/L10e family
Structure domains:
36-MER Chain: B
Molecule details ›
Chain: B
Length: 36 nucleotides
Theoretical weight: 11.63 KDa
Source organism: Thermus thermophilus
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand K 1 x K
Ligand BU1 1 x BU1
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P6522
Unit cell:
a: 67.87Å b: 67.87Å c: 340.47Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.212 0.247
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 citation in other articles

Effects of Noncanonical Base Pairing on RNA Folding: Structural Context and Spatial Arrangements of G·A Pairs.
Olson et al. (2019)
5 more

13 mentions without citation

Computational approaches to 3D modeling of RNA.
Laing et al. (2010)
12 more