› 2hxd

X-ray diffraction
2.3Å resolution

Bifunctional dCTP deaminase-dUTPase mutant enzyme variant E145A from Methanocaldococcus jannaschii in complex with alpha,beta-imido dUTP and magnesium

Released:
DOI: 10.2210/pdb2hxd/pdb
PDB entry 2hxd coloured by chain, front view.
PDB entry 2hxd coloured by chain, side view.
PDB entry 2hxd coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Entry authors: Bynck JH, Johansson E

Function and Biology Details

Reaction catalysed: 
dCTP + 2 H(2)O = dUMP + diphosphate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
homo 24-mer
Assembly name:
PDBe Complex ID:
PDB-CPX-176493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dCTP deaminase, dUMP-forming Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.4 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57872 (Residues: 1-204; Coverage: 100%)
Gene names: MJ0430, dcd
Sequence domains: dCTP deaminase-like
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand DUP 1 x DUP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I432
Unit cell:
a: 171.513Å b: 171.513Å c: 171.513Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.221
Expression system: Escherichia coli BL21(DE3)

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