› 2hzl

X-ray diffraction
1.4Å resolution

Crystal structures of a sodium-alpha-keto acid binding subunit from a TRAP transporter in its closed forms

Released:
DOI: 10.2210/pdb2hzl/pdb
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Source organism: Cereibacter sphaeroides 2.4.1
Primary publication:
Crystal structures of an Extracytoplasmic Solute Receptor from a TRAP transporter in its open and closed forms reveal a helix-swapped dimer requiring a cation for alpha-keto acid binding.
Gonin S, Arnoux P, Pierru B, Lavergne J, Alonso B, Sabaty M, Pignol D
OpenAccess logo BMC Struct Biol 7 11 (2007)
PMID: 17362499

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174606 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-keto acid-binding periplasmic protein TakP Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 40.02 KDa
Source organism: Cereibacter sphaeroides 2.4.1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q3J1R2 (Residues: 1-365; Coverage: 100%)
Gene names: RHOS4_17040, RSP_0097, smoM, takP
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand PYR 3 x PYR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 118.021Å b: 78.057Å c: 95.139Å
α: 90° β: 124.98° γ: 90°
R-values:
R R work R free
0.173 0.173 0.184
Expression system: Escherichia coli BL21

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Citations

6 review citations

A structural classification of substrate-binding proteins.
Berntsson et al. (2010)
5 more

5 mentions without citation

Enhancing UCSF Chimera through web services.
Huang et al. (2014)
4 more

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