› 2i4z

X-ray diffraction
2.25Å resolution

Crystal structure of the complex between PPARgamma and the partial agonist LT127 (ureidofibrate derivative). This structure has been obtained from crystals soaked for 6 hours.

Released:
DOI: 10.2210/pdb2i4z/pdb
PDB entry 2i4z coloured by chain, front view.
PDB entry 2i4z coloured by chain, side view.
PDB entry 2i4z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into the mechanism of partial agonism: crystal structures of the peroxisome proliferator-activated receptor gamma ligand-binding domain in the complex with two enantiomeric ligands.
Pochetti G, Godio C, Mitro N, Caruso D, Galmozzi A, Scurati S, Loiodice F, Fracchiolla G, Tortorella P, Laghezza A, Lavecchia A, Novellino E, Mazza F, Crestani M
J Biol Chem 282 17314-24 (2007)
PMID: 17403688

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisome proliferator-activated receptor gamma Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 32.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37231 (Residues: 223-504; Coverage: 56%)
Gene names: NR1C3, PPARG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

1 bound ligand:
Ligand DRH 1 x DRH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2
Unit cell:
a: 93Å b: 60.14Å c: 118.08Å
α: 90° β: 103.7° γ: 90°
R-values:
R R work R free
0.238 0.238 0.29
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Signaling Mechanisms of Selective PPARγ Modulators in Alzheimer's Disease.
Govindarajulu et al. (2018)
3 more

5 mentions without citation

Review of the Structural and Dynamic Mechanisms of PPARγ Partial Agonism.
Kroker et al. (2015)
4 more