› 2if9

X-ray diffraction
2.59Å resolution

Crystal Structure of SV40 T-antigen origin binding domain disulfide-linked dimer

Released:
DOI: 10.2210/pdb2if9/pdb
PDB entry 2if9 coloured by chain, front view.
PDB entry 2if9 coloured by chain, side view.
PDB entry 2if9 coloured by chain, top view.
Source organism: Macaca mulatta polyomavirus 1
Primary publication:
The crystal structure of the SV40 T-antigen origin binding domain in complex with DNA.
Meinke G, Phelan P, Moine S, Bochkareva E, Bochkarev A, Bullock PA, Bohm A
OpenAccess logo PLoS Biol 5 e23 (2007)
PMID: 17253903

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189169 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large T antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15.41 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q98ZP7 (Residues: 131-260; Coverage: 24%)
Sequence domains: Origin of replication binding protein
Structure domains: Replication Protein E1; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 68.463Å b: 63.982Å c: 63.228Å
α: 90° β: 94.45° γ: 90°
R-values:
R R work R free
0.213 0.208 0.296
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

The Mcm complex: unwinding the mechanism of a replicative helicase.
Bochman et al. (2009)
10 more

5 mentions without citation

The critical protein interactions and structures that elicit growth deregulation in cancer and viral replication.
Ou et al. (2011)
4 more