› 2imi

X-ray diffraction
1.4Å resolution

Structures of an Insect Epsilon-class Glutathione S-transferase from the Malaria Vector Anopheles Gambiae: Evidence for High DDT-detoxifying Activity

Released:
DOI: 10.2210/pdb2imi/pdb
PDB entry 2imi coloured by chain, front view.
PDB entry 2imi coloured by chain, side view.
PDB entry 2imi coloured by chain, top view.
Source organism: Anopheles gambiae
Primary publication:
Structure of an insect epsilon class glutathione S-transferase from the malaria vector Anopheles gambiae provides an explanation for the high DDT-detoxifying activity.
Wang Y, Qiu L, Ranson H, Lumjuan N, Hemingway J, Setzer WN, Meehan EJ, Chen L
J Struct Biol 164 228-35 (2008)
PMID: 18778777

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181873 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glutathione transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 24.88 KDa
Source organism: Anopheles gambiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7PVS6 (Residues: 1-219; Coverage: 99%)
Gene names: 1280155, AgaP_AGAP009194, GSTE2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 49.065Å b: 83.793Å c: 92.488Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.193
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Genotype to phenotype, the molecular and physiological dimensions of resistance in arthropods.
Feyereisen et al. (2015)
2 more

8 mentions without citation

A single mutation in the GSTe2 gene allows tracking of metabolically based insecticide resistance in a major malaria vector.
Riveron et al. (2014)
7 more