› 2iwt

X-ray diffraction
2.3Å resolution

Thioredoxin h2 (HvTrxh2) in a mixed disulfide complex with the target protein BASI

Released:
DOI: 10.2210/pdb2iwt/pdb
PDB entry 2iwt coloured by chain, front view.
PDB entry 2iwt coloured by chain, side view.
PDB entry 2iwt coloured by chain, top view.
Source organism: Hordeum vulgare
Primary publication:
Structural basis for target protein recognition by the protein disulfide reductase thioredoxin.
Maeda K, Hägglund P, Finnie C, Svensson B, Henriksen A
Structure 14 1701-10 (2006)
PMID: 17098195

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139651 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thioredoxin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.45 KDa
Source organism: Hordeum vulgare
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7XZK2 (Residues: 1-122; Coverage: 100%)
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin
Alpha-amylase/subtilisin inhibitor Chain: B
Molecule details ›
Chain: B
Length: 189 amino acids
Theoretical weight: 20.92 KDa
Source organism: Hordeum vulgare
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07596 (Residues: 21-203; Coverage: 100%)
Sequence domains: Trypsin and protease inhibitor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

1 bound ligand:
Ligand FLC 2 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P41212
Unit cell:
a: 76.148Å b: 76.148Å c: 129.432Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Fluorescent protein-based redox probes.
Meyer et al. (2010)
13 more

11 mentions without citation

The structure of the bacterial oxidoreductase enzyme DsbA in complex with a peptide reveals a basis for substrate specificity in the catalytic cycle of DsbA enzymes.
Paxman et al. (2009)
10 more