› 2ixf

X-ray diffraction
2Å resolution

Crystal structure of the ATPase domain of TAP1 with ATP (D645Q, Q678H mutant)

Released:
DOI: 10.2210/pdb2ixf/pdb
PDB entry 2ixf coloured by chain, front view.
PDB entry 2ixf coloured by chain, side view.
PDB entry 2ixf coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Distinct structural and functional properties of the ATPase sites in an asymmetric ABC transporter.
Procko E, Ferrin-O'Connell I, Ng SL, Gaudet R
Mol Cell 24 51-62 (2006)
PMID: 17018292

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + antigen peptide(Side 1) = ADP + phosphate + antigen peptide(Side 2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153179 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antigen peptide transporter 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 271 amino acids
Theoretical weight: 29.51 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36370 (Residues: 465-725; Coverage: 36%)
Gene names: Abcb2, Mtp1, Tap1
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand ATP 4 x ATP
Ligand MG 4 x MG
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 82.269Å b: 108.917Å c: 123.561Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
22 more

3 mentions without citation

Inhibition of multidrug resistance-linked P-glycoprotein (ABCB1) function by 5'-fluorosulfonylbenzoyl 5'-adenosine: evidence for an ATP analogue that interacts with both drug-substrate-and nucleotide-binding sites.
Ohnuma et al. (2011)
2 more