› 2j5l

X-ray diffraction
2.9Å resolution

Structure of a Plasmodium falciparum apical membrane antigen 1-Fab F8. 12.19 complex

Released:
DOI: 10.2210/pdb2j5l/pdb
PDB entry 2j5l coloured by chain, front view.
PDB entry 2j5l coloured by chain, side view.
PDB entry 2j5l coloured by chain, top view.
Source organisms:
Primary publication:
Cross-reactivity studies of an anti-Plasmodium vivax apical membrane antigen 1 monoclonal antibody: binding and structural characterisation.
Igonet S, Vulliez-Le Normand B, Faure G, Riottot MM, Kocken CH, Thomas AW, Bentley GA
J Mol Biol 366 1523-37 (2007)
PMID: 17229439

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-212326 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Apical membrane antigen 1 Chain: A
Molecule details ›
Chain: A
Length: 581 amino acids
Theoretical weight: 67.04 KDa
Source organism: Plasmodium falciparum
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9BIM8 (Residues: 1-402; Coverage: 100%)
Gene name: ama-1
Sequence domains: Apical membrane antigen 1
Ig-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 213 amino acids
Theoretical weight: 23.29 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q5XFY8 (Residues: 23-235; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins
FAB FRAGMENT OF MONOCLONAL ANTIBODY F8.12.19 Chain: C
Molecule details ›
Chain: C
Length: 225 amino acids
Theoretical weight: 24.19 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P63
Unit cell:
a: 171.9Å b: 171.9Å c: 44.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.212 0.278
Expression system: Komagataella pastoris

Quick links

Citations

8 review citations

Plasmodium vivax: who cares?
Galinski et al. (2008)
7 more

3 mentions without citation

The apicomplexan glideosome and adhesins - Structures and function.
Boucher et al. (2015)
2 more