2j98

X-ray diffraction
1.8Å resolution

Human coronavirus 229E non structural protein 9 cys69ala mutant (Nsp9)

Released:

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-143095 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Viral protein genome-linked nsp9 Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12.03 KDa
Source organism: Human coronavirus 229E
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C6X1 (Residues: 3825-3933; Coverage: 2%)
Gene names: 1a-1b, rep
Sequence domains: Coronavirus replicase NSP9
Structure domains: Replicase NSP9
Viral protein genome-linked nsp9 Chain: B
Molecule details ›
Chain: B
Length: 109 amino acids
Theoretical weight: 12.03 KDa
Source organism: Human coronavirus 229E
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C6X1 (Residues: 3825-3933; Coverage: 2%)
Gene names: 1a-1b, rep
Sequence domains: Coronavirus replicase NSP9
Structure domains: Replicase NSP9

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P212121
Unit cell:
a: 26.396Å b: 61.377Å c: 107.309Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.214 0.278
Expression system: Escherichia coli