› 2jec

X-ray diffraction
2Å resolution

crystal structure of recombinant DiocleA grandiflora lectin mutant E123A-H131N-K132Q complexed witH 5-bromo-4-chloro-3-indolyl-a-D- mannose

Released:
DOI: 10.2210/pdb2jec/pdb
PDB entry 2jec coloured by chain, front view.
PDB entry 2jec coloured by chain, side view.
PDB entry 2jec coloured by chain, top view.
Source organism: Macropsychanthus grandiflorus
Primary publication:
Insights into the structural basis of the pH-dependent dimer-tetramer equilibrium through crystallographic analysis of recombinant Diocleinae lectins.
Nagano CS, Calvete JJ, Barettino D, Pérez A, Cavada BS, Sanz L
Biochem J 409 417-28 (2008)
PMID: 17937659

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lectin alpha chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 239 amino acids
Theoretical weight: 25.75 KDa
Source organism: Macropsychanthus grandiflorus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08902 (Residues: 1-237; Coverage: 100%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand MN 8 x MN
Ligand XMM 4 x XMM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P212121
Unit cell:
a: 72.194Å b: 84.552Å c: 176.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.179 0.23
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

ConA-Like Lectins: High Similarity Proteins as Models to Study Structure/Biological Activities Relationships.
Cavada et al. (2018)
1 more