› 2jf6

X-ray diffraction
2.82Å resolution

Structure of inactive mutant of Strictosidine Glucosidase in complex with strictosidine

Released:
DOI: 10.2210/pdb2jf6/pdb
PDB entry 2jf6 coloured by chain, front view.
PDB entry 2jf6 coloured by chain, side view.
PDB entry 2jf6 coloured by chain, top view.
Source organism: Rauvolfia serpentina
Primary publication:
Molecular architecture of strictosidine glucosidase: the gateway to the biosynthesis of the monoterpenoid indole alkaloid family.
Barleben L, Panjikar S, Ruppert M, Koepke J, Stöckigt J
Plant Cell 19 2886-97 (2007)
PMID: 17890378

Function and Biology Details

Reaction catalysed: 
Strictosidine + H(2)O = D-glucose + strictosidine aglycone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Strictosidine-O-beta-D-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 532 amino acids
Theoretical weight: 60.96 KDa
Source organism: Rauvolfia serpentina
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GU20 (Residues: 1-532; Coverage: 100%)
Gene name: SGR1
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand S55 2 x S55
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P4212
Unit cell:
a: 159Å b: 159Å c: 102.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.28
Expression system: Escherichia coli

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Citations

11 review citations

Alkaloid biosynthesis: metabolism and trafficking.
Ziegler et al. (2008)
10 more

3 mentions without citation

A microbial supply chain for production of the anti-cancer drug vinblastine.
Zhang et al. (2022)
2 more