2jhj

X-ray diffraction
1.9Å resolution

3-methyladenine dna-glycosylase from Archaeoglobus fulgidus

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-127986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HhH-GPD domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 295 amino acids
Theoretical weight: 34.3 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28163 (Residues: 1-295; Coverage: 100%)
Gene name: AF_2117
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 69.89Å b: 48.85Å c: 104.46Å
α: 90° β: 106.07° γ: 90°
R-values:
R R work R free
0.184 0.181 0.239
Expression system: Escherichia coli BL21(DE3)