› 2jkz

X-ray diffraction
3.45Å resolution

SACCHAROMYCES CEREVISIAE HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE IN COMPLEX WITH GMP (GUANOSINE 5'- MONOPHOSPHATE) (ORTHORHOMBIC CRYSTAL FORM)

Released:
DOI: 10.2210/pdb2jkz/pdb
PDB entry 2jkz coloured by chain, front view.
PDB entry 2jkz coloured by chain, side view.
PDB entry 2jkz coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Functional significance of four successive glycine residues in the pyrophosphate binding loop of fungal 6-oxopurine phosphoribosyltransferases.
Moynié L, Giraud MF, Breton A, Boissier F, Daignan-Fornier B, Dautant A
Protein Sci 21 1185-96 (2012)
PMID: 22610485

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170001 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine-guanine phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 220 amino acids
Theoretical weight: 25.09 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04178 (Residues: 2-221; Coverage: 100%)
Gene names: BRA6, D9509.18, HPT1, YDR399W
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand 5GP 4 x 5GP
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21212
Unit cell:
a: 118.077Å b: 176.362Å c: 91.109Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Molecular Recognition of Lipid II by Lantibiotics: Synthesis and Conformational Studies of Analogues of Nisin and Mutacin Rings A and B.
Dickman et al. (2019)
4 more

1 mention without citation

Functional significance of four successive glycine residues in the pyrophosphate binding loop of fungal 6-oxopurine phosphoribosyltransferases.
Moynié et al. (2012)