Function and Biology Details
Reaction catalysed:
Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- PtsI, HPr-binding domain superfamily
- PEP-utilising enzyme, conserved site
- PEP-utilising enzyme, C-terminal
- Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily
- Pyruvate kinase-like domain superfamily
- PEP-utilising enzyme, mobile domain
- Phosphohistidine domain superfamily
- Phosphotransferase system, enzyme I N-terminal
2 more domains
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
Phosphoenolpyruvate-protein phosphotransferase (preferred)
PDBe Complex ID:
PDB-CPX-140395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate-protein phosphotransferase
Molecule details ›
Chains: A, B
Length: 573 amino acids
Theoretical weight: 63.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 573 amino acids
Theoretical weight: 63.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P08839 (Residues: 1-573; Coverage: 100%)
Sequence domains:
- PEP-utilising enzyme, N-terminal
- PEP-utilising enzyme, mobile domain
- PEP-utilising enzyme, PEP-binding domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Experimental Method:
Solution NMR, X-ray solution scattering
Expression system: Escherichia coli BL21(DE3)
