› 2m2u

Solution NMR

Binary complex of African Swine Fever Virus Pol X with MgdGTP

Released:
DOI: 10.2210/pdb2m2u/pdb
PDB entry 2m2u coloured by chain, ensemble of 20 models, front view.
PDB entry 2m2u coloured by chain, ensemble of 20 models, side view.
PDB entry 2m2u coloured by chain, ensemble of 20 models, top view.
Source organism: African swine fever virus
Primary publication:
How a low-fidelity DNA polymerase chooses non-Watson-Crick from Watson-Crick incorporation.
Wu WJ, Su MI, Wu JL, Kumar S, Lim LH, Wang CW, Nelissen FH, Chen MC, Doreleijers JF, Wijmenga SS, Tsai MD
J Am Chem Soc 136 4927-37 (2014)
PMID: 24617852

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154656 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Repair DNA polymerase X Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20.35 KDa
Source organism: African swine fever virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P42494 (Residues: 1-174; Coverage: 100%)
Gene names: Ba71V-97, O174L
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand DGT 1 x DGT
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 88%
Refinement method: distance geometry
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Stable isotope labeling methods for DNA.
Nelissen et al. (2016)
10 other articles

1 mention without citation

In silico identification of multi-target inhibitors from medicinal fungal metabolites against the base excision repair pathway proteins of African swine fever virus.
Macalalad et al. (2024)