› 2mrf

Solution NMR

NMR structure of the ubiquitin-binding zinc finger (UBZ) domain from human Rad18

Released:
DOI: 10.2210/pdb2mrf/pdb
PDB entry 2mrf coloured by chain, ensemble of 20 models, front view.
PDB entry 2mrf coloured by chain, ensemble of 20 models, side view.
PDB entry 2mrf coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
NMR structure of the human Rad18 zinc finger in complex with ubiquitin defines a class of UBZ domains in proteins linked to the DNA damage response.
Rizzo AA, Salerno PE, Bezsonova I, Korzhnev DM
Biochemistry 53 5895-906 (2014)
PMID: 25162118

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase RAD18 Chain: A
Molecule details ›
Chain: A
Length: 33 amino acids
Theoretical weight: 3.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NS91 (Residues: 198-227; Coverage: 6%)
Gene names: RAD18, RNF73

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 88%
Refinement method: simulated annealing/torsian angle dynamics, molecular dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Control of DNA Damage Bypass by Ubiquitylation of PCNA.
Ripley et al. (2020)
4 more

1 mention without citation

A Comprehensive Survey of the Roles of Highly Disordered Proteins in Type 2 Diabetes.
Du et al. (2017)