2msd

Solution NMR

NMR data-driven model of GTPase KRas-GNP tethered to a lipid-bilayer nanodisc

Released:

Function and Biology Details

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-133993 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Truncated apolipoprotein A-I Chains: A, C
Molecule details ›
Chains: A, C
Length: 200 amino acids
Theoretical weight: 23.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02647 (Residues: 68-265; Coverage: 80%)
Gene name: APOA1
Sequence domains: Apolipoprotein A1/A4/E domain
GTPase KRas Chain: B
Molecule details ›
Chain: B
Length: 187 amino acids
Theoretical weight: 21.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-186; Coverage: 98%)
  • Best match: P01116-2 (Residues: 1-185)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 0%
Refinement method: simulated annealing
Expression system: Escherichia coli