› 2msp

X-ray diffraction
3.3Å resolution

MAJOR SPERM PROTEIN, BETA ISOFORM, ENGINEERED C59S/T90C MUTANT, PUTATIVE SUBFILAMENT STRUCTURE, PH 8.5

Released:
DOI: 10.2210/pdb2msp/pdb
PDB entry 2msp coloured by chain, front view.
PDB entry 2msp coloured by chain, side view.
PDB entry 2msp coloured by chain, top view.
Source organism: Ascaris suum
Primary publication:
Structural basis for amoeboid motility in nematode sperm.
Bullock TL, McCoy AJ, Kent HM, Roberts TM, Stewart M
Nat Struct Biol 5 184-9 (1998)
PMID: 9501910

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151005 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major sperm protein isoform beta Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 126 amino acids
Theoretical weight: 14.12 KDa
Source organism: Ascaris suum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27440 (Residues: 2-127; Coverage: 99%)
Sequence domains: MSP (Major sperm protein) domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P41212
Unit cell:
a: 79.66Å b: 79.66Å c: 463.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The VAP protein family: from cellular functions to motor neuron disease.
Lev et al. (2008)
2 more

1 mention without citation

A systematic review of exercise studies for individuals hospitalized with an acute exacerbation of chronic obstructive pulmonary disease: Focus on the principles of exercise training.
Moecke et al. (2023)