2my3

Solution NMR

Snu17p-Pml1p structure intermediate during RES complex assembly

Released:
Primary publication:
Structures of intermediates during RES complex assembly.
OpenAccess logo Sci Rep 5 12545 (2015)
PMID: 26212312

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-154146 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
U2 snRNP component IST3 Chain: A
Molecule details ›
Chain: A
Length: 118 amino acids
Theoretical weight: 13.5 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40565 (Residues: 25-138; Coverage: 77%)
Gene names: IST3, SNU17, YIB5W, YIR005W
Sequence domains: RNA recognition motif
Pre-mRNA leakage protein 1 Chain: B
Molecule details ›
Chain: B
Length: 23 amino acids
Theoretical weight: 2.51 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07930 (Residues: 22-42; Coverage: 10%)
Gene names: L1591, PML1, YLR016C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)