› 2ntl

X-ray diffraction
2.6Å resolution

Crystal structure of PurO/AICAR from Methanothermobacter thermoautotrophicus

Released:
DOI: 10.2210/pdb2ntl/pdb
PDB entry 2ntl coloured by chain, front view.
PDB entry 2ntl coloured by chain, side view.
PDB entry 2ntl coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
A novel function for the N-terminal nucleophile hydrolase fold demonstrated by the structure of an archaeal inosine monophosphate cyclohydrolase.
Kang YN, Tran A, White RH, Ealick SE
Biochemistry 46 5050-62 (2007)
PMID: 17407260

Function and Biology Details

Reaction catalysed: 
IMP + H(2)O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
IMP cyclohydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 222 amino acids
Theoretical weight: 24.03 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O27099 (Residues: 1-202; Coverage: 100%)
Gene names: MTH_1020, purO
Sequence domains: IMP cyclohydrolase-like protein
Structure domains: Inosine monophosphate cyclohydrolase-like

Ligands and Environments

1 bound ligand:
Ligand AMZ 4 x AMZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P32
Unit cell:
a: 85.913Å b: 85.913Å c: 124.29Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.208 0.243
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
3 more

3 mentions without citation

Detection of ligand binding hot spots on protein surfaces via fragment-based methods: application to DJ-1 and glucocerebrosidase.
Landon et al. (2009)
2 more