› 2nv0

X-ray diffraction
1.73Å resolution

Structure of the glutaminase subunit Pdx2 (YaaE) of PLP synthase from Bacillus subtilis

Released:
DOI: 10.2210/pdb2nv0/pdb
PDB entry 2nv0 coloured by chain, front view.
PDB entry 2nv0 coloured by chain, side view.
PDB entry 2nv0 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structure of a bacterial pyridoxal 5'-phosphate synthase complex.
Strohmeier M, Raschle T, Mazurkiewicz J, Rippe K, Sinning I, Fitzpatrick TB, Tews I
Proc Natl Acad Sci U S A 103 19284-9 (2006)
PMID: 17159152

Function and Biology Details

Reactions catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153422 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyridoxal 5'-phosphate synthase subunit PdxT Chains: A, B
Molecule details ›
Chains: A, B
Length: 196 amino acids
Theoretical weight: 21.47 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P37528 (Residues: 1-196; Coverage: 100%)
Gene names: BSU00120, pdxT, yaaE
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 45.44Å b: 81.606Å c: 117.285Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.216
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Vitamin B6: a molecule for human health?
Hellmann et al. (2010)
13 more

5 mentions without citation

Structural Dynamics and Perspectives of Vitamin B6 Biosynthesis Enzymes in Plasmodium: Advances and Open Questions.
Barra et al. (2021)
4 more